欧美在线视频免费观看_亚洲欧美日韩一区二区_国语成人_综合精品久久_不卡一二三区_亚洲国产精品自拍

咨詢熱線

15000266580

當(dāng)前位置:首頁(yè) >產(chǎn)品中心>細(xì)胞庫(kù)>動(dòng)物卵巢細(xì)胞>CRL-9618CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

簡(jiǎn)要描述:CRL-9618 CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株,ATCC 細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞|細(xì)胞|貼壁細(xì)胞|懸浮細(xì)胞|,細(xì)胞庫(kù)管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和*培養(yǎng)條件

  • 產(chǎn)品型號(hào):CRL-9618
  • 廠商性質(zhì):生產(chǎn)廠家
  • 更新時(shí)間:2025-05-25
  • 訪  問(wèn)  量:2892

產(chǎn)品分類

Product Category

詳細(xì)介紹

CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

1957年,Puck TT從成年中國(guó)倉(cāng)鼠卵巢的活檢組織建立了CHO細(xì)胞,CHO-K1是CHO的一個(gè)亞克隆。CHO-K1的生長(zhǎng)需要

培養(yǎng)條件:F12K培養(yǎng)基(SIGMA,貨號(hào)N3520,添加NaHCO3 2.5g/L),90%;優(yōu)質(zhì)胎牛血清,10%


動(dòng)物種別:中國(guó)倉(cāng)鼠

性別:雌

組織來(lái)源:卵巢

形態(tài):上皮細(xì)胞

傳代方法的介紹   1:4-1:8

支原體檢測(cè)   陰性

以下是此細(xì)胞ATCC

CHO-K1 (ATCC® CCL-61™)
CHO-K1 倉(cāng)鼠卵巢細(xì)胞亞株

Organism Cricetulus griseus, hamster, Chinese

Tissue  ovary

Product Format  frozen

Morphology  epithelial-like

Culture Properties  adherent

Biosafety Level  1

Gender  female

Applications

This cell line is suitable as a transfection host.

Storage Conditions  liquid nitrogen vapor phase

Karyotype  Chromosome Frequency Distribution 50 Cells: 2n = 22. Stemline number is hypodiploid.

Derivation

The CHO-K1 cell line was derived as a subclone from the parental CHO cell line initiated from a biopsy of an ovary of an adult Chinese hamster by T. T. Puck in 1957.

Clinical Data

female

Virus Susceptibility  Vesicular stomatitis, Orsay (Indiana)

Vesicular stomatitis, Glasgow (Indiana)

Getah virus

Virus Resistance

poliovirus 2; modoc virus; Button Willow virus

Comments

The cells require proline in the medium for growth.

Complete Growth Medium  The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing

Volumes are given for a 75 cm2 flask. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w

) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:4 to 1:8 is recommended

Medium Renewal: Once or twice between subculture

Cryopreservation

Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Temperature: 37°C





















產(chǎn)品咨詢

留言框

  • 產(chǎn)品:

  • 您的單位:

  • 您的姓名:

  • 聯(lián)系電話:

  • 常用郵箱:

  • 省份:

  • 詳細(xì)地址:

  • 補(bǔ)充說(shuō)明:

  • 驗(yàn)證碼:

    請(qǐng)輸入計(jì)算結(jié)果(填寫(xiě)阿拉伯?dāng)?shù)字),如:三加四=7
聯(lián)系方式

郵箱:[email protected]

地址:上海市虹口區(qū)四平路710號(hào)7層

咨詢熱線

400-821-8510

(周一至周日9:00- 19:00)

在線咨詢
  • 掃一掃 微信咨詢

Copyright©2025 上海復(fù)祥生物科技有限公司 All Right Reserved    備案號(hào):滬ICP備10013034號(hào)-2     sitemap.xml
技術(shù)支持:化工儀器網(wǎng)    管理登陸
主站蜘蛛池模板: 伊人www| 国产精品久久久久久久久久免费看 | 日韩在线观看一区二区 | 免费的黄色片子 | 日韩经典一区 | 国产精品久久片 | 日韩免费在线视频 | 午夜免费观看视频 | 亚洲精品电影在线一区 | 日本在线观看视频一区 | 在线成人av| 色小妹一二三区 | 久久久久久久久久久久国产精品 | 欧美一级二级三级视频 | 中文字幕 视频一区 | 欧美日韩一区二区三区在线观看 | 青青草久草 | 亚洲免费资源 | 91精品视频在线播放 | 午夜免费| 亚洲www啪成人一区二区 | 福利二区 | 久久成人国产 | 国产精品久久久久久久久久久久久 | 国产成人精品一区二 | 国产精品视频专区 | 99re视频在线观看 | 黄色一级片视频播放 | 在线观看国产一级片 | 国产成人精品一区二区 | 日韩精品视频在线观看免费 | 欧美国产精品一区二区 | 国产一区日韩在线 | 成人免费黄色片 | 搜一级毛片 | 日本私人网站在线观看 | 日韩色视频 | 天天插天天操天天干 | 国产日产精品一区二区三区四区 | 91在线入口 | 国产精品伦理一区 |